A RT-qPCR system using a degenerate probe for specific identification and differentiation of SARS-CoV-2 Omicron (B.1.1.529) variants of concernArticle Published on 2022-10-052022-11-15 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] B.1.1.529 B.1.617.2 circulating complement degenerate Delta delta variant detect dominant Inclusion introduced L452R Mutation offer omicron pathogenic platform primer probe probe sequence probes replaced resource RT-qPCR RT-qPCRs SARS-CoV-2 SARS-CoV-2 variant Sequencing Spread Surveillance the spike protein variant variants variants of concern whole genome [DOI] 10.1371/journal.pone.0274889 PMC 바로가기
Electrochemical genosensor for the specific detection of SARS-CoV-2SARS-CoV-2의 특정 검출을 위한 전기화학적 제노센서Article Published on 2022-08-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] alternative biotinylated capture caused cell cultures complex complexes coronavirus disease COVID-19 cultures detect Diagnosis diagnostic discrimination disease DNA-RNA followed by Genosensor homologous horseradish peroxidase imperative in vitro Increases limit of detection maleimide molecular mortality rate Nanobioconjugate pandemic Particles peroxidase probe probes respiratory respiratory viruses responsible SARS-CoV-2 SARS-CoV-2 cell SARS-CoV-2 detection SARS-CoV-2. sensitivity serological test Serological tests Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 Signaling streptavidin Surveillance tested the patient virus [DOI] 10.1016/j.talanta.2022.123482 PMC 바로가기 [Article Type] Article
Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variantsSARS-CoV-2 변이체 식별을 위한 새로운 RT-qPCR 용융 곡선 분석 분석 사용의 검증 및 이점Article Published on 2022-07-292022-09-11 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 진단, 치료기술, [키워드] clinical monitoring Cohort complementary Contact tracing diagnosing E484K examined FIVE gold gold standard in viral K417N melting curve analysis Mutation mutations N439K N501Y new virus P681H positive probes quantitative PCR ranged reverse transcription risk stratification RT-qPCR Sample Sanger sequencing SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike glycoprotein SARS-CoV-2 variant sensitivity Sensitivity and specificity sequence significantly specificity spike glycoprotein Spread the SARS-CoV-2 Transcription validation validation cohort variant variants VOCs [DOI] 10.1038/s41598-022-17339-0 PMC 바로가기 [Article Type] Article
Probing the mutation independent interaction of DNA probes with SARS-CoV-2 variants through a combination of surface-enhanced Raman scattering and machine learning표면 강화 라만 산란 및 기계 학습의 조합을 통해 SARS-CoV-2 변이체와 DNA 프로브의 돌연변이 독립적 상호작용 조사Article Published on 2022-07-152022-09-11 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료기술, 치료법, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus antigenicity antisense Antisense oligonucleotide Antisense oligonucleotides ASO binding efficiency Characteristics characterized clinically Combination Concentration coronavirus described detect develop diagnostic diagnostic test DNA Evolution Genetic human population hypothetical Immune profile independent Interaction mechanism mutate Mutation Mutation resistant probe mutations N gene negative sample nucleocapsid Nucleocapsid phosphoprotein Point mutation positive probe probes Probing reveal RNA SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 variant SARS-CoV-2 variants SARS-CoV-2 virus scattering Selective and ultrasensitive diagnosis sensitivity sequence severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 specificity surface-Enhanced Raman scattering. technique the SARS-CoV-2 virus therapeutic Transmissibility unique variants virus [DOI] 10.1016/j.bios.2022.114200 PMC 바로가기 [Article Type] Article
A Universal Fluorescent Immunochromatography Assay Based on Quantum Dot Nanoparticles for the Rapid Detection of Specific Antibodies against SARS-CoV-2 Nucleocapsid ProteinSARS-CoV-2 뉴클레오캡시드 단백질에 대한 특정 항체의 신속한 검출을 위한 양자점 나노입자 기반 범용 형광 면역크로마토그래피 분석Article Published on 2022-06-022022-09-11 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, [키워드] acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 antibody complex Concentration conditions conserved coronavirus coronavirus 2 coupling COVID-19 detection Diagnosis diagnostic Dot expansion expressed Fluorescence fluorescent fluorescent immunochromatography assay Host IgG immunochromatography life cycle limit of detection Mutation rates nanoparticle Ni-NTA nucleocapsid nucleocapsid protein optimization pathogenic point of care point-of-care Point-of-care detection probes Protein protein A purified purity quantum quantum dots. Rapid related viruses respiratory respiratory infection SARS-CoV-2 SARS-CoV-2-specific antibodies SARS-CoV-2-specific antibody serological Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus shown Specific specific antibodies specific antibody specificity Spike protein Spike proteins susceptible Transmission Universal virus virus viruses was determined zoonotic coronavirus zoonotic coronaviruses [DOI] 10.3390/ijms23116225 PMC 바로가기 [Article Type] Article
Simultaneous detection of SARS-CoV-2 and identification of spike D614G mutation using point-of-care real-time polymerase chain reaction현장 진료 실시간 중합효소 연쇄반응을 이용한 SARS-CoV-2 동시 검출 및 스파이크 D614G 돌연변이 식별Article Published on 2022-06-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus Analysis applied Chain Reaction contagiousness coronavirus COVID-19 D614G D614G mutation detect Detection sensitivity detection system Diagnosis equipment high mortality Human labeling Laboratory Molecular diagnosis Multiple multiplex Mutation PCR platform POCT point-of-care polymerase chain polymerase chain reaction probe probes rapid detection method Real-time PCR respiratory virus respiratory viruses reverse transcription PCR rRT-PCR SARS-CoV-2 SARS-CoV-2 mutant SARS-CoV-2 mutants Sensitivity and specificity severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 Simultaneous specificity spike Spike D614G mutation. strain was reduced wild type [DOI] 10.1016/j.jviromet.2022.114513 PMC 바로가기 [Article Type] Article
Development of a Test System to Detect the Omicron Variant of SARS-CoV-2 and the Frequency of Its Detection in PatientsSARS-CoV-2의 오미크론 변이체 및 환자의 검출 빈도를 감지하는 테스트 시스템 개발Article Published on 2022-06-012022-09-11 Journal: Bulletin of experimental biology and medicine [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] allele allele-specific reverse transcription PCR (AS-RT-PCR) B.1.1.529 clinical clinical samples collected contagiousness coronavirus detect detection development dominant exceptional first test Frequency Genome Genotype less Mutation mutations omicron Omicron variant omicron variant. Patient patients primer probe probes Region reverse transcription PCR RT-PCR SARS-CoV SARS-CoV 2 SARS-CoV-2 System Test [DOI] 10.1007/s10517-022-05524-0 PMC 바로가기 [Article Type] Article
Simultaneous analysis of antigen-specific B and T cells after SARS-CoV-2 infection and vaccinationSARS-CoV-2 감염 및 예방접종 후 항원특이 B 세포와 T 세포 동시 분석Article Published on 2022-06-012022-09-11 Journal: Cytometry. Part A : the journal of the Internation [Category] COVID19(2023년), SARS, 치료기술, [키워드] Activation Analysis analyzed Antigen antigen-specific B cell B cells cells Conventional deplete Effect facilitate flow cytometry immune profiling independent assay independent assays lymphocyte Lymphocytes marker mRNA vaccination natural infection platform Population probes reactive reduced SARS-CoV-2 SARS-COV-2 infection significantly Simultaneous specimens spike Spike protein T and B cells T cell T cell subset T cells technique vaccination vaccination. [DOI] 10.1002/cyto.a.24563 PMC 바로가기 [Article Type] Article
Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through OmicronSARS-CoV-2 변이체에 대한 스파이크 엑토도메인 및 그 하위 도메인의 분자 프로브, 알파부터 오미크론까지Article Published on 2022-05-242022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] Addgene Alpha Amino acid amino acid sequence antibody antigenicity Beta binding antibodies Biotin C-terminal characterization characterized circulating variants cleavage convalescent plasma COVID-19 convalescent donors COVID-19 pandemic Delta displaying dissemination ectodomain Effectiveness Epsilon expressing facilitate Gamma Human immune responses Immunity Infection Iota Isolation Lambda matrix molecular monoclonal antibodies Mutation N-terminal N-terminal domain Neutralizing antibodies neutralizing antibody nine NTD omicron outbreak physical physical properties plasma antibody probe probes produced protease purification RBD Receptor-binding domain region of interest SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant SARS-CoV-2 variants sequence sequences serum antibody specificity the RBD the receptor-binding domain the SARS-CoV-2 Vaccine variant variant spike variants widespread yeast [DOI] 10.1371/journal.pone.0268767 PMC 바로가기 [Article Type] Article
Establishment of an in-house real-time RT-PCR assay for the detection of severe acute respiratory syndrome coronavirus 2 using the first World Health Organization international standard in a resource-limited country자원이 제한된 국가에서 최초의 세계보건기구 국제표준을 이용한 중증급성호흡기증후군 코로나바이러스 2 검출을 위한 사내 실시간 RT-PCR 분석법 구축Article Published on 2022-05-012022-09-11 Journal: Journal of Clinical Laboratory Analysis [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome caused Clinical performance clinical sample clinical samples conserved coronavirus correlation country COVID-19 COVID-19 pandemic Critical E gene evaluated house International international standard limit of detection molecular molecular assays multiplex N gene Nasopharyngeal samples Nasopharyngeal swab nasopharyngeal swabs NIBSC Patient PCR assay positive Primers probe probes public health real-time RT-PCR real-time RT-PCR. reference standard Region RT-PCR assay Saliva SARS-CoV-2 specimen Standard Target genes WHO World Health Organization [DOI] 10.1002/jcla.24355 PMC 바로가기 [Article Type] Article